Named for the iconic 'Star Trek' spacecraft, the Enterprise was the first of NASA's fleet of space shuttles.
Cheers and thunderous applause erupted from the crowd of New Yorkers and tourists who turned out to see the retired spacecraft moved to its new home atop the flight deck of repurposed World War Two aircraft carrier now named the Intrepid Sea, Air & Space Museum.
"Beam me up, Scotty!" a man in the crowd shouted as a crane lifted the shuttle off a barge onto the museum, repeating a catch phrase from the popular science fiction television show.
"It's part of our history," said New Yorker Cameron Fisher, 21.
The crowd grew quiet and the air was filled with the creaking of the crane and beating of a helicopter circling overhead as the shuttle rose 230 feet into the air.
"It inspires people to see what human ingenuity can achieve," said British tourist Kirsty Rushen, 35.
Earlier Wednesday, as the shuttle, strapped to a barge, made its way up the Hudson River and past the Statue of Liberty, crowds ranging from small children to elderly couples strained for a glimpse of the craft.