The story of young love helped lure female viewers, who made up 42 percent of the audience, according to Sony. Seventy-three percent of reviews compiled on website Rotten Tomatoes recommended the movie.
"This is a film that had something for women," said Rory Bruer, president of worldwide distribution for Sony Pictures Entertainment. "Gwen Stacy is a pretty strong woman in her own right, and the two of them had great chemistry on screen."
The new "Spider-Man" cost about $215 million to produce, plus tens of millions more for marketing. The film is Sony's second big-budget hit this summer, following the success of alien comedy "Men in Black 3" in May.
The studio's next major release, sci-fi remake "Total Recall," reaches theaters in August.
Last week's winner, comedy "Ted" about a man and his foul-mouthed, pot-smoking teddy bear, slipped to second place with domestic sales of $32.6 million from Friday through Sunday. Directed by "Family Guy" creator Seth MacFarlane, "Ted" has earned $120.2 million domestically since its debut a week ago.